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Aabra Ka Dabra (2004) L, Agra Ka Daabra (2004) 720p, Aabra Ka Daabra (2004) MP4, Aabra Ka Daabra (2004) HD,Biphasic effects of morphine upon c-fos expression in locus coeruleus and periaqueductal gray of the rat.
The effects of an injection of morphine (10 mg/kg i.p.) upon c-fos expression within the locus coeruleus (LC) and the nucleus of the solitary tract (NTS), periaqueductal gray (PAG) have been studied in rats using a highly sensitive quantitative in situ hybridization procedure. The c-fos mRNA was visualized using digoxigenin labeled, non-isotopic probes. Morphine induced c-fos expression in the LC, but not the NTS, in a time-dependent manner. Following a 10 min exposure to morphine, c-fos RNA was increased up to 6-fold in the LC. In contrast, following a 30 min exposure to morphine, c-fos RNA was decreased in the LC. These effects of morphine in the LC were virtually identical to those seen in the dorsal motor nucleus of the vagus in the same temporal sequence. Morphine had no effect on c-fos expression in the NTS. In both the LC and the NTS, morphine induced a marked increase in c-fos RNA expression 6-24 h following drug treatment, without a subsequent decrease in expression. These results suggest that the opioid antagonist, naloxone, blocks the morphine-induced decrement in c-fos expression in the LC. Together, these results show that morphine has a distinct and dose-related biphasic effect upon c-fos expression within the LC and suggest that morphine may act in the LC to modulate the expression of c-fos in this brain region.//
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